Emerging hybrid, experimental/computational approaches to cellular dosimetry can be used by particle toxicologists to accurately calculate the delivered dose to cells for various particles and under different in vitro experimental conditions as a function of exposure time. Likewise, in vivo lung dosimetry models allow researchers to estimate the delivered particle dose in any region of the respiratory system, as well as study the implications of particle properties and breathing parameters for diverse animal species. Moreover, knowing the deposited dose will also facilitate the extrapolation from experimental animals (rat, mouse, rabbit, pig and monkey) to humans of all ages. Most importantly, incorporating such dosimetric methodologies in the study design enables particle toxicologists to bring in vitro and in vivo doses to the same scale, an important step towards the development and validation of in vitro cellular screening assays. Dosimetric modelling of deposition requires input of several aerosol characteristics, including density, and modelling retention involves knowledge about particle bio-dissolution. For both in vivo (inhalation) and in vitro (cell cultures) studies knowledge about Exposure-Dose-Response relationships is key for comparing in vitro and in vivo results on an equal dosimetric basis, and at the same time it provides an opportunity to validate in vitro assays. Ultimately, a careful attention to dosimetric details allows a scientifically justified risk extrapolation of toxicological results from animal studies to humans.
The Seminar is free entry. The request of a certificate and / or declaration of attendance must be ordered on the day of the event at the ISPUP Secretariat.
Professor Flemming Cassee is an Inhalation Toxicologist at The National Institute for Public Health and Environment (RIVM), The Netherlands. In this position he is involved in research into adverse health effects from airborne particulate matter (fine dust) and gaseous components (e.g. ozone, nitrogen dioxide) in the ambient air. In addition to this, Flemming Cassee is Professor of Inhalation Toxicology at the Institute of Risk Assessment Sciences of the Utrecht University, The Netherlands. More recently his focus is also on the safety of nanomaterials. He is advisor to the Dutch Ministry of Infrastructure and the Environment, the Health Council of the Netherlands, WHO and EU, US EPA and the American Health Effects Institute, and others. Furthermore, he is Editor-in-Chief of the journal Particle and Fibre Toxicology and a reviewer/editor of a number of international scientific journals.